Journal: International Journal of Molecular Sciences
Article Title: Lamivudine, Doravirine, and Cabotegravir Downregulate the Expression of Human Endogenous Retroviruses (HERVs), Inhibit Cell Growth, and Reduce Invasive Capability in Melanoma Cell Lines
doi: 10.3390/ijms25031615
Figure Lengend Snippet: Concentration-dependent reduction of cell viability after antiretroviral treatments. Cell viability was assessed using the Sulforhodamine B (SRB) assay after 72 h of treatment with antiretroviral drugs in four human melanoma cell lines (SK-Mel-28, A375, FO-1, and MeWo), as well as in normal human epithelial melanocytes (NHEM). Dacarbazine, a medication commonly used in the treatment of melanoma, was employed for comparison. Data were acquired by calculating the mean ± S.D. of values from at least four independent experiments, which were then compared with the untreated control; * p < 0.05; ** p < 0.01.
Article Snippet: A375 (CRL-1619) and FO-1 (CRL-12177) melanoma cell lines and Normal Human Epithelial Melanocytes (NHEM, PCS-200-013) obtained from ATCC (Manassas, VA, USA) were cultured at 37 °C in a 5% CO 2 humidified atmosphere.
Techniques: Concentration Assay, Sulforhodamine B Assay, Comparison, Control